Zhiyong Liu, Ph.D.

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Scientists&Research

Zhiyong Liu, Ph.D.

Associate Investigator, NIBS, Beijing

Phone: 010-80726688-8551
Email: liuzhiyong@qfwhyh.com

Research Description

Our research will focus on three interrelated directions: (1) The molecular and genetic mechanisms underlying auditory system development; (2) Hearing restoration in various deafness mouse models; (3) Multi-omics analysis of different 购宝钱包 types and subtypes in the auditory system.

(1) The molecular and genetic mechanisms underlying auditory system development

The cochlea is the peripheral sound-detecting organ and contains four main 购宝钱包 types: 1) Outer hair 购宝钱包 s (OHCs) that are sound amplifiers; 2) Inner hair 购宝钱包 s (IHCs) that are primary sensory 购宝钱包 s and decode sound information; 3) Supporting 购宝钱包 s (SCs): crucial for maintaining cochlear integrity and serving as a resource for IHC and OHC regeneration; 4) Spiral ganglion neurons (SGNs) that form synapses with IHCs and project sound information to the brain. Ototoxic factors, including genetic mutations, antibiotics, chemotherapy drugs, and aging, can cause degeneration of OHCs, IHCs, and SGNs, leading to hearing impairment. According to the World Health Organization (WHO) report, 0.3% of newborns, 5% of individuals under 45, and 50% of those over 70 suffer from varying degrees of hearing loss. More importantly, hearing deficits not only affect sound perception but also hinder social interaction.

The cochlea develops from posterior placode progenitors (PPP), which give rise to otic placode (vesicle) or epibranchial placode 购宝钱包 s. The ventral portion of the otic vesicle ultimately transforms into the cochlea, where neurosensory progenitors diverge into neural or sensory progenitors. Neural progenitors develop into SGNs, while sensory progenitors differentiate into OHCs, IHCs, and SCs. The key questions we aim to address include:

1) How are otic vesicle progenitors specified from the general PPP?

2) How do otic neurosensory progenitors adopt neural or sensory fates?

3) How do cochlear progenitors commit to OHC, IHC, or SC lineages?

Briefly, our long-term research goal is to identify the key genes or gene networks that regulate the proliferation and differentiation of otic progenitors before they commit to the aforementioned 购宝钱包 types. This will undoubtedly contribute to future hearing restoration efforts.

(2) Hearing restoration in deafness mouse models

Non-mammalian vertebrates such as birds and fish can restore hearing after trauma by transdifferentiating SCs into hair 购宝钱包 s. In contrast, mammals, including mice and humans, lack this regenerative capacity. As a result, the degeneration of hair 购宝钱包 s in mammals inevitably leads to irreversible hearing impairment or complete hearing loss. Therefore, achieving hearing restoration in mammals is a critical area of research. To better mimic human deafness, our group has developed distinct deafness mouse models, in which OHCs, IHCs, or SGNs are selectively damaged. Furthermore, we have successfully reprogrammed SCs into IHCs or OHCs by combining Atoh1 with Tbx2, and Atoh1 with Ikzf2, respectively. However, these newly regenerated hair 购宝钱包 s fail to restore hearing because they are not fully differentiated or matured.

What are the key barriers preventing hearing recovery in damaged cochleae? Our data suggest that although the ribbon synaptic structures in the newly regenerated IHCs and OHCs are well-formed, their stereocilia (or hair bundles) are dysfunctional. Stereocilia are where the mechanoelectrical (MET) components are located, and defects in stereocilia prevent those newly regenerated hair 购宝钱包 s from detecting sound. Identifying the factors critical for regulating stereocilia assembly, as well as discovering genes that, in combination with Atoh1, Tbx2, or Ikzf2, can produce fully functional hair 购宝钱包 s with well-developed stereocilia, is essential for improving hearing restoration in damaged cochleae.

(3) Multi-omics analysis of different 购宝钱包 types or subtypes in auditory system

Historically, cochlear 购宝钱包 types have been classified based on , morphology, and function, which facilitates communication across research groups but leads to significant heterogeneity within the “same” 购宝钱包 type. This is one of the main reasons why multi-omics analysis of cochlear 购宝钱包 types often lacks high resolution. Our ultimate goal is to develop a suite of mouse genetic tools, including but not limited to CreER strains, to label 购宝钱包 subtypes at a much higher resolution. Our group has already obtained RNA-seq and ATAC-seq datasets from various cochlear 购宝钱包 types and subtypes. However, these datasets represent an initial version, as the 购宝钱包 subtype specificity is currently of low resolution. To improve this, we will employ high-throughput in situ hybridization to identify potential 购宝钱包 subtype-specific genes. Genes with sparse expression patterns in vivo, and exclusive expression in one 购宝钱包 subtype, will be prioritized for generating mouse knock-in genetic tools.

These new genetic tools will enable us to sort young 购宝钱包 s using FACS or manually isolate adult 购宝钱包 s. Approximately 500–1,000 pure 购宝钱包 s from each subtype will be pooled for bulk RNA-seq and ATAC-seq. This will provide a much deeper dataset with a higher signal-to-noise ratio compared to single-购宝钱包 analysis. We anticipate obtaining the second generation of high-depth, 购宝钱包 subtype-specific gene data and chromatin open regions, which will reveal key cis-regulatory elements (e.g., promoters and enhancers). These subtype-specific promoters and enhancers will be valuable for generating 购宝钱包 type-specific adeno-associated viruses (AAVs), which can deliver wild-type genes to treat deaf patients with corresponding genetic mutations. For example, Gjb2, a high-incidence deafness gene in the Chinese population and expressed in SCs, will be a focus. We aim to generate pan-SC or SC subtype-specific AAVs for therapeutic applications.

Representative Publication

1) Li C*, Zhao Y*, Wang L*, Li S*, Li J, Gu Y, He S, Wang G, Lei F, Lu Y, Gu L, Xu S, Xiong W, Li B^#, Liu Z^#. Rbm24 promotes outer hair 购宝钱包 survival through Insm1 repression while independently regulating hair bundle morphogenesis. Development, 2026;153(8): dev204234. doi: 10.1242/dev.204234. ^#co-correspondence

2) Sun, Y., Ren, M., Zhang, Y., Li, S., Luo, Z., Sun, S., He, S., Wang, G., Zhang, D., Mansour, SL., Song, L., Liu, Z. Casz1 is required for both inner hair 购宝钱包 fate stabilization and outer hair 购宝钱包 survival. Science, 2025 Jan 30: eado4930. doi: 10.1126/science.ado4930.

3) Hao, S., Zhu, X., Huang, Z., Zhang, H.… Liu, Z.^#, Liu, L.^#, Liu, S.^#, Sun, Y.^# and Liu, C.^#Cross-species single-购宝钱包 spatial transcriptomic atlases of the cerebellar cortex. Science, 2024; 385(6716): eado3927. ^#co-correspondence

4) Bi, Z., Ren M., Zhang Y., He S., Song L., Li X^#, Liu Z^#. Revisiting the potency of Tbx2 expression in transforming outer hair 购宝钱包 s into inner hair 购宝钱包 s at multiple ages in vivo. The Journal of Neuroscience, 2024; 44(23): e1751232024. ^#co-correspondence

5) Wang, G, Gu, Y. Liu, Z., Deciphering the genetic interactions between Pou4f3, Gfi1 and Rbm24 in maintaining cochlear hair 购宝钱包 survival. eLife, 2024; 14:12:RP90025.

6) Li, X., Ren, M., Gu, Y., Zhu, T., Zhang, Y., Li, J., Li, C., Wang, G., Song, L., Bi, Z^#., Liu, Z.^# In situ regeneration of inner hair 购宝钱包 s in the damaged cochlea by temporally regulated coexpression of Atoh1 and Tbx2. Development, 2023;150(24): dev201888. ^#co-correspondence

7) Chen, A., Sun, Y., Lei, Y., Li, C...... Liu, Z.^#, Xu, X.^#and Li, C.^#. Single-购宝钱包 spatial transcriptome reveals 购宝钱包 -type organization in macaque cortex. 购宝钱包 , 2023;186(17):3726-3743. ^#co-correspondence

8) Li, S., He S, Lu Y, Jia S, Liu, Z. Epistatic genetic interactions between Insm1 and Ikzf2 during cochlear outer hair 购宝钱包 development. 购宝钱包 Reports, 2023; 42(5):112504.

9) Luo, Z*., Du Y*., Li, S., Zhang H., Shu M., Zhang D., He S., Wang G., Lu F.^#, Liu, Z.^# Three distinct Atoh1 enhancers cooperate for sound receptor hair 购宝钱包 development. PNAS, 2022; 119(32): e2119850119. ^#co-correspondence

10) Bi, Z*., Li, X*., Ren, M*., Gu, Y., Zhu T., Li, S. Wang G., Sun S., Sun Y., Liu, Z. Development and transdifferentiation into inner hair 购宝钱包 s require Tbx2. National Science Review, 2022; 9(12): nwac156.

11) Sun, Y*., Wang, L*., Zhu, T., Wu, B., Wang, G., Luo, Z., Li, C^#., Wei, W^#., Liu, Z.^# Single-购宝钱包 transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages. 购宝钱包 Reports, 2022; 38: 110542.^#co-correspondence

12) Sun S*., Li S*., Luo Z*., Ren M., He S., Wang G., Liu Z.Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting 购宝钱包 s into outer hair 购宝钱包 s. eLife, 2021;10: e66547.

13) Wang G*., Li C*., He S., Liu Z. Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Development, 2021;148(5): dev196899.

14) Li C*, Li X*, Bi Z*, Sugino K, Wang G, Liu Z. Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages. eLife, 2020; 9: e50491.

15) Zhang H*, Pan H*, Zhou C*, Wei Y, Ying W, Li, S, Wang G, Li, C, Ren Y, Li G, Ding X, Sun Y, Li G, Song L, Li Y, Yang H^#, Liu Z^#. Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Development. 2018; 145(20): pii: dev168906. ^#co-correspondence

16) Liu Z*, Yang C*, Sugino K*, Fu C*, Liu L, Yao X, Luke P. L, Lee T. Opposing intrinsic temporal gradients guide neural stem 购宝钱包 production of varied neuronal fates. Science. 2015; 350 (6258): 317-320. *co-first author

Review

Sun Y., Liu Z. Recent advancements in molecular studies of cochlear development and regeneration. Current Opinion in Neurobiology, 2023; 81:102745.